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Biological System

Microbiome Composition

Shifts in gut and skin microbial communities

Overview

The gut microbiome — trillions of bacteria, fungi, and other microorganisms — influences immunity, metabolism, and even cognition. In the closed environment of a spacecraft, microbial communities shift. The Inspiration4 metagenomics studies profiled stool (OSD-630), skin swabs from 10 body sites (OSD-572), and environmental capsule surfaces (OSD-573) across 8 timepoints.

Data sources: OSD-572 (skin swabs, 10 body sites), OSD-573 (Dragon capsule surfaces), OSD-630 (stool metagenome) — Metaphlan taxonomy + pathway abundances

Key findings

  • Gut microbiome composition (OSD-630) showed individual-specific trajectories; no single taxon shifted consistently across all crew.
  • Skin microbiome (OSD-572) reflected the shared capsule environment — some site-specific convergence was observed.
  • Capsule surface microbiome (OSD-573) tracked crew skin and oral communities, consistent with prior ISS research.
  • Pathway abundance data (HUMAnN3) is used here rather than raw species counts for interpretability.
n=4 crew members · Individual differences dominate the signal

Individual trajectories

fold-change from L-44 baseline · n=4

Pathway abundance fold-change from pre-flight median. n=4 for gut; capsule samples reflect environmental, not individual, data.

C001Commander

Microbiome Composition · C001
Trajectory chart renders after preprocessing

C002Pilot

Microbiome Composition · C002
Trajectory chart renders after preprocessing

C003Medical Officer

Microbiome Composition · C003
Trajectory chart renders after preprocessing

C004Mission Specialist

Microbiome Composition · C004
Trajectory chart renders after preprocessing

Related dashboard domains

How to read this page: These findings describe four individuals at one point in time. No finding should be generalized to spaceflight health broadly. Individual differences dominate the signal. Fold-changes labeled “2× individual baseline” use derived thresholds, not clinical cutoffs.