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Biological System

Immune & Inflammatory Response

How the body's defense system shifts during spaceflight

Overview

The immune system is the body's defense network — a complex web of cells and proteins that identify and neutralize threats. During spaceflight, this system undergoes measurable shifts. The Inspiration4 crew showed changes in circulating cytokine levels, white blood cell composition, and T/B-cell receptor repertoire diversity, all measured at multiple timepoints before, during (proxied by R+1), and after the 3-day mission.

Data sources: OSD-570 VDJ repertoire (TCR/BCR), PBMC snRNA-seq cell type ratios, OSD-575 71-plex cytokine panel (Alamar + Eve panels)

Key findings

  • Cytokine levels (TNFα, IL-6, IL-1β) showed individual-specific trajectories — no uniform crew-wide pattern.
  • TCR clonal diversity, measured via VDJ repertoire sequencing, shifted acutely at R+1 and began recovering by R+45.
  • WBC and neutrophil counts tracked similarly to known short-duration spaceflight patterns.
  • All observations reflect direction of change from each individual's own L-44 baseline — not a population reference.
n=4 crew members · Individual differences dominate the signal

Individual trajectories

fold-change from L-44 baseline · n=4

Lines show fold-change from each crew member's L-44 baseline. Shaded band = typical reference range from OSD-575 data files.

C001Commander

Immune & Inflammatory Response · C001
Trajectory chart renders after preprocessing

C002Pilot

Immune & Inflammatory Response · C002
Trajectory chart renders after preprocessing

C003Medical Officer

Immune & Inflammatory Response · C003
Trajectory chart renders after preprocessing

C004Mission Specialist

Immune & Inflammatory Response · C004
Trajectory chart renders after preprocessing

Related dashboard domains

How to read this page: These findings describe four individuals at one point in time. No finding should be generalized to spaceflight health broadly. Individual differences dominate the signal. Fold-changes labeled “2× individual baseline” use derived thresholds, not clinical cutoffs.